320 µg/L (3w)
mixed leptotene / zygoteneAfter exposure to tamoxifen, there is dissynchrony of spermatogenesis, i.e. spermatogenic cells of subsequent stages occur within a single spermatogenic cyst (right, compare to control, left). This change appears to be dose-dependent.
All images were taken from adult male zebrafish; H&E staining.
mixed leptotene / zygotene / pachytene
mixed leptotene / spermatid
adult male zebrafish exposed to 320 µg tamoxifen /L for 3 weeks (lower image); reference in upper image. H&E staining. After exposure to tamoxifen (bottom), the interstitium of the testis has a distended, edematous appearance, compared to the control testis (top). This feature is associated with the presence of dilated capillaries.
This distended interstitium contains large clusters of interstitial Leydig cells, in contrast to the control animal, where these Leydig cells mostly occur singly or in small clusters.
Similar large clusters of Leydig cells were found after exposure to the anti-androgen flutamide.